Isoamyl Acetate Protocol is appropriate when wearing an organic vapor respirator. If used to fit test particulate respirators, the respirator must be equipped with an organic vapor filter. The odor of the isoamyl acetate (banana oil) can be detected at low levels by the user, making it a simple, effective test.

Saccharin Solution Aerosol method determines if the individual being tested can detect the taste of saccharin. This is a common test when particulate filter masks are used, but it may not be as effective as other methods since only a sweet taste response is elicited.

Bitrex (denatonium benzoate) uses the same protocol as the saccharin test, but a bitter taste is elicited instead. Bitrex is routinely used as a taste aversion agent in household liquids that children should not be drinking.

Irritant Smoke uses a person's response to the irritating chemicals released in the vapor produced by a stannic chloride ventilation smoke tube to detect leakage into the respirator. It is designed for respirators equipped with high efficiency particulate air (HEPA) or P100 filters. The smoke can be irritating to the eyes, lungs, and nasal passages, so the person conducting the test should minimize the subject's exposure to the irritant smoke.

Generated Aerosol QNFT utilizes an aerosol mixture administered in a test chamber. It utilizes a sampling port on the test specimen respirator into which a probe is inserted to detect the concentration differential. The expense and technical requirements of this method make it less desirable for most employers.

The Ambient Aerosol Condensation Nuclei Counter also fit tests respirators with the use of a probe. The Portacount Fit Test requirements are similar to the previous method, but are easier to administer because it is a portable device available from some equipment rental firms.

The Controlled Negative Pressure fit testing protocol is an alternative to aerosol fit-test methods in that it is a computerized testing device that uses air pressure to perform the testing.